Sample Submisssion Form

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Automated DNA Sequencing at The University of Idaho

(Part I must be completed!)

Part I
Name:
Department:
Name of PI if different:
phone:
fax:
date:
time:
email address:
budget #
Select service requested Service A, User supplied completed reactions. $5.00 Service B, User supplied plasmid DNA, we do the sequencing reactions. $35.00 Service C, User supplied E. coli containing plasmid plus service B. $50.00 Service D, User supplied PCR product (with priming site). One direction (service B) $30.00 Service E. "Difficult DNA (eg. high GC content). User supplied E. coli. Includes sevice A. Both directions. $40.00 Service F, Primer walking. Inquire.

List of sample identifiers:
Number of templates in this order?
How do you want your data?	text file (GCG format) SCF
Part II
Length of insert or PCR product to be sequenced	bp
Form of DNA	dsDNA ssDNA
PCR product	yes no
Strain of bacterium used to produce DNA
Plasmid/phagemid used
M13
Lambda
Cosmid
Purification procedure (check one)	organic extraction Column (type) CsCL gradient Other
Concentration of DNA (ug or pmol used)
Method used to determine concentration	gel electrophoresis ethidium plate spectrophotometer fluorimeter
Method used to do sequencing reactions	Cycle sequencing using SequiTherm Sequenase / dye-dATP other
Primers used/or to be used?	M13F M13R KS SK SP6 T7 T3 other

http://www.uidaho.edu/pses/sequencer/